1.Choosing the right cell line
When choosing the appropriate cell line for your experiment, please consider the following criteria:
a.Species: Non-human and non-primate cell lines usually have fewer biosafety restrictions, but in the end your experiment will determine whether to use a culture of a particular species.
b.Features: What is the purpose of your experiment? For example, cell lines derived from liver and kidney may be more suitable for toxicity testing.
c.Limited or continuous: Although choosing from a limited cell line may provide you with more options for expressing the correct function, continuous cell lines are generally easier to clone and maintain.
d.Normal or transformed: Transformed cell lines usually have a higher growth rate and higher seeding efficiency, are continuous, and require less serum in the culture medium, but their phenotype has undergone permanent changes through genetic transformation.
e.Growth conditions and characteristics: What are your requirements for growth speed, saturation density, cloning efficiency and suspension growth ability? For example, to express recombinant proteins in high yields, you may need to select cell lines that have fast growth rates and the ability to grow in suspension.
f.Other criteria: If you are using a limited cell line, is there enough stock available? Is the cell line fully characterized, or do you have to verify it yourself? If you are using an abnormal cell line, is there an equivalent normal cell line that can be used as a control? Is the cell line stable? If not, how easy is it to clone it and generate enough frozen stock for your experiment?
2.Obtain cell lines
You can build your own culture from primary cells, or you can choose to purchase established cell cultures from commercial or non-profit suppliers (ie cell banks). Reputable suppliers provide high-quality cell lines that have been carefully tested for integrity and ensure that the culture is free of contaminants. We recommend not to borrow cultures from other laboratories because they have a high risk of cell culture contamination. Regardless of its source, please make sure all new cell lines have been tested for mycoplasma contamination before you start using it.
Post time: Feb-01-2023